林木蛋白质组学研究

内容简介

针对蛋白质组学研究热点、难点问题，《林木蛋白质组学研究》主要汇聚了《林木蛋白质组学研究》组近年来在林木蛋白组学方面的研究进展和部分成果。主要内容包括蛋白质组学在林木生长发育、非生物逆境胁迫（干旱、高温、低温）、适应性进化等方面的研究内容。

目录

PREFACE
Chapter 1:Advances in Proteomics Research on Forest Tree
　1.1　Introduction
　1.2　Research progress on proteomic technology system
　1.3　The question in current proteomics research
　1.4　Proteomics applications on forestry
　1.5　Conclusion and prospect
　References
Chapter 2:Comparison of Methods for Protein Extraction from Pine Needles
　2.1　Introduction
　2.2　Materials and methods
　2.3　Results
　2.4　Discussion
　References
Chapter 3:Temporal and Spatial Profiling of Internod Elongation-Associated Protein
　expression in Rapidly Growing Culms of Bamboo
　3.1　Introduction
　3.2　Materials and methods
　3.3　Results
　3.4　Discussion
　3.5　Conclusion
　References
Chapter 4: Physiological and Protein Responses to Drought in Four Pine Seedlings
　4.1　Introduction
　4.2　Material and methods
　4.3　Results and Discussion
　References
Chapter 5:Proteins Responding to Drought and High-Temperature Stress in Pinus armandii Franch
　5.1　Introduction
　5.2　Material and methods
　5.3　Results and Discussion
　5.4　Concluding remarks
　References
Chapter 6:Proteins Responding to Drought and High-Temperature Stress in Populus x euramericana cv. '74/76'
　6.1　Introduction
　6.2　Materials and methods
　6.3　Results
　6.4　Discussion
　6.5　Conclusions remarks
　References
Chapter 7:Physiological and Protein Responses to Drought Stress in Hippophae rhamnoides
　7.1　Introduction
　7.2　Material and method
　7.3　Results
　7.4　Discussion
　7.5　Conclusion
　References
Chapter 8:Clonal Reproduction and Natural Variation of Populus canescens Patches
　8.1　Introduction
　8.2　Materials and methods
　8.3　Results
　8.4　Discussion
　References
Color Plates

摘要与插图

Chapter 1 Advances in Proteomics Research on Forest Tree

Abstract: Forest plays an important role in maintenance of ecosystem equilibrium and provides material energy. It has a set of mechanism of its own growth, development, and metabolism. Proteomics is a crucial means to reveal the material life phenomenon in biology nowadays. In this chapter, the trend of technical system of proteomics, such as extraction, separation, identification method of protein, was introduced and existing problem was indicated. In addition, different research direction of forest proteomics (i.e. tress response, wood formation, developmental regulation, pest control) were discussed respectively combined with overseas research. Finally, the prospect to its future development was given.
1.1 Introduction
With the advent of the post-genome era, proteomics have been a rapid development. Compared with genomics, proteomics can provide some unparalleled advantages. Firstly, it more accurately identifies the proteins and obtains related functions. Secondly, the “one gene, one protein (or function)” hypothesis is thus not applicable at present time, to a certain extent, proteomics can detect post translational modifications (PTMs) (Agrawal and Rakwal, 2006). Although proteomics research has been used on a large number of species, it mainly concentrated on model species and animals. about less than 5% of species was plant which focused on model plants such as Arabidopsis (Arabidopsis thaliana), wheat (Triticum aestivum), rice (Oryza sativa) and maize (Zea mays), poplar (Populus trichocarpa), etc. (Jorrín-Novo et al., 2009; Oeljeklaus et al., 2009). The species involved in the forest were less, which is disaccord with forestry importance in the ecosystem. Due to global climate change, energy shortages, the contradictions between human and nature will stand out in the future. Forestry role in human life will strengthen increasingly. So, it is a very meaningful work to spread proteomics research on forest trees to solve production problems. In this section, the research progress of proteomic technology system and hot research topics are being reviewed. Finally, the prospect of proteomics research in the forestry is discussed.
1.2 Research progress on proteomic technology system
1.2.1 Protein extraction method
Extraction of high concentration and high purity protein is the most important in the process of the whole proteomics research. Because proteins include many complex biochemical properties, such as charge number, molecular weight, hydrophobicity, hydrophilicity, post-translational modification, and interaction with other molecules, and these properties are changed along with the species difference, such as developmental stages, cell and tissue types, and different growth conditions, many protein extraction methods were established. Harvesting the maximum amount of protein to match protein analysis (s